Enhancing Nutritional and Antioxidant Properties of Breadsticks through germinated and Non-germinated Cowpea flours
DOI:
https://doi.org/10.57252/jrpfoods.2026.1Keywords:
Cowpea, germination, bioactive properties, Antioxidant activity, sensory evaluationAbstract
This study evaluates the impact of incorporation of whole wheat flour with germinated and non-germinated cowpea flours (12 % addition) on textural, sensorial, and physicochemical properties of breadsticks. Incorporation of germinated cowpea flour in breadsticks exhibited significantly highest total phenolic content (5.61 mg Gallic acid/g) and antioxidant activity (93 %), compared to non-germinated cowpea (78 %) and whole wheat flour (76.9 %). The inclusion of germinated cowpea flour significantly enhanced protein content while reducing carbohydrates and fat content. Hardness and the crispness were also significantly influenced by incorporation of cowpea flours. Moreover, germinated breadstick achieved the highest hedonic scores in terms of appearance, overall acceptability, and taste by semi-trained panelists. During 120 days of storage, germinated cowpea breadsticks retained better colour, taste and texture due to reduced moisture uptake. Therefore, germinated cowpea flour in breadsticks could be an antioxidant enriched alternative, without causing substantial changes in product quality and acceptability, by 12% addition of whole wheat flour.
References
Afify, A. E. M. M. R., El-Beltagi, H. S., El-Salam, S. M. A., & Omran, A. A. (2012). Effect of Soaking, Cooking, Germination and Fermentation Processing on Proximate Analysis and Mineral Content of Three White Sorghum Varieties (Sorghum bicolor L. Moench). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2), 92–98. https://doi.org/10.15835/NBHA4027930
Ahsan, M., Moin, A., Ashraf, H., Kamran, M., Manzoor, I., & Giuffrè, A. M. (2023). Technological, quality and nutritional characteristics of Ramen noodles with wheat flour partially substituted by water chestnut flour. Italian Journal of Food Science, 35(4), 136–146.
Ahsan, M., Moin, A., Ashraf, H., Khan, A., & Giuffrè, A. M. (2024). Formulation and characterization of reduced fat muffins using a plant based fat replacer. Journal of Food Science and Technology, 62(3), 551–561.
Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8(4), 96. https://doi.org/10.3390/plants8040096
AlOudat, M., Magyar, N., Simon-Sarkadi, L., & Lugasi, A. (2021). Nutritional content of ready-to-eat meals sold in groceries in Hungary. International Journal of Gastronomy and Food Science, 24, 100318. https://doi.org/10.1016/J.IJGFS.2021.100318
Barman, A., Marak, C. M., Sangma, R. M. B. and C. S., Barman, A., Marak, C. M., & Sangma, R. M. B. and C. S. (2018). Nutraceutical Properties of Legume Seeds and Their Impact on Human Health. Legume Seed Nutraceutical Research. https://doi.org/10.5772/INTECHOPEN.78799
Bryant, C. J. (2022). Plant-based animal product alternatives are healthier and more environmentally sustainable than animal products. Future Foods, 6, 100174. https://doi.org/10.1016/J.FUFO.2022.100174
Chung, H. J., Cho, A., & Lim, S. T. (2014). Utilization of germinated and heat-moisture treated brown rices in sugar-snap cookies. LWT - Food Science and Technology, 57(1), 260–266. https://doi.org/10.1016/J.LWT.2014.01.018
Cornejo, F., Caceres, P. J., Martínez-Villaluenga, C., Rosell, C. M., & Frias, J. (2015). Effects of germination on the nutritive value and bioactive compounds of brown rice breads. Food Chemistry, 173, 298–304. https://doi.org/10.1016/J.FOODCHEM.2014.10.037
Desire, M. F., Blessing, M., Elijah, N., Ronald, M., Agather, K., Tapiwa, Z., Florence, M. R., & George, N. (2021). Exploring food fortification potential of neglected legume and oil seed crops for improving food and nutrition security among smallholder farming communities: A systematic review. Journal of Agriculture and Food Research, 3, 100117. https://doi.org/10.1016/J.JAFR.2021.100117
Dur-e-Shahwar, S., Tahira, M. A., Tanveer, A., & Abid, H. (2018). Textural, Bioactive and Sensory Attributes of Breadsticks Containing Germinated and Non-Germinated Legumes. 4(3).
Ghavidel, R. A., & Prakash, J. (2007). The impact of germination and dehulling on nutrients, antinutrients, in vitro iron and calcium bioavailability and in vitro starch and protein digestibility of some legume seeds. LWT - Food Science and Technology, 40(7), 1292–1299. https://doi.org/10.1016/J.LWT.2006.08.002
Guzmán-Ortiz, F. A., Castro-Rosas, J., Gómez-Aldapa, C. A., Mora-Escobedo, R., Rojas-León, A., Rodríguez-Marín, M. L., Falfán-Cortés, R. N., & Román-Gutiérrez, A. D. (2019). Enzyme activity during germination of different cereals: A review. Food Reviews International, 35(3), 177–200. https://doi.org/10.1080/87559129.2018.1514623;WGROUP:STRING:PUBLICATION
Hiran, P., Kerdchoechuen, O., & Laohakunjit, N. (2016). Combined effects of fermentation and germination on nutritional compositions, functional properties and volatiles of maize seeds. Journal of Cereal Science, 71, 207–216. https://doi.org/10.1016/J.JCS.2016.09.001
International, A. (2000). Official methods of analysis of AOAC International (Vol. 17, Issues 1–2). AOAC international.
Jaworska, D., & Hoffmann, M. (2008). Relative importance of texture properties in the sensory quality and acceptance of commercial crispy products. Journal of the Science of Food and Agriculture, 88(10), 1804–1812. https://doi.org/10.1002/JSFA.3283;ISSUE:ISSUE:DOI
Jian, G. X., Cheng, R. T., Qing, P. H., Ji, Y. L., Xiang, D. W., & Xiang, D. T. (2009). Dynamic changes in phenolic compounds and antioxidant activity in oats (Avena nuda L.) during steeping and germination. Journal of Agricultural and Food Chemistry, 57(21), 10392–10398.
https://doi.org/10.1021/JF902778J
Kaushik, G., Satya, S., & Naik, S. N. (2010). Effect of domestic processing techniques on the nutritional quality of the soybean. Mediterranean Journal of Nutrition and Metabolism, 3(1), 39–46. https://doi.org/10.3233/S12349-009-0079-7
Keawkim, K., Lorjaroenphon, Y., Vangnai, K., & Jom, K. N. (2021). Metabolite–Flavor Profile, Phenolic Content, and Antioxidant Activity Changes in Sacha Inchi (Plukenetia volubilis L.) Seeds during Germination. Foods 2021, Vol. 10, Page 2476, 10(10), 2476. https://doi.org/10.3390/FOODS10102476
Latimer, G. W. (Ed.). (2019). Official methods of analysis of AOAC International (19th ed.). AOAC International.
Lin, P. Y., & Lai, H. M. (2006). Bioactive compounds in legumes and their germinated products. Journal of Agricultural and Food Chemistry, 54(11), 3807–3814. https://doi.org/10.1021/JF060002O
Ma, A., Li, R., Bai, T., Sun, Y., Wang, L., Hou, D., & Zhou, S. (2025). Gluten-free whole mung bean noodles: Role of transglutaminase and hydrocolloids in their physicochemical, sensory, and in vitro starch digestion characteristics. Journal of Future Foods. https://doi.org/10.1016/J.JFUTFO.2025.09.035
M. Rehman., Enzyme Activities and degradation of anti nutrients in chicpea (Cicer Arietinium L.,)seeds during germination. J.Bio-Sci, 16, 29-34 (2008)
Moin, A., Ali, T. M., & Hasnain, A. (2019). Effect of basmati and irri acetylated rice starches on textural and sensorial characteristics of dumpling wrappers. Journal of Food Measurement and Characterization, 13(4). https://doi.org/10.1007/s11694-019-00179-4
Moin, A., Ali, T. M., & Hasnain, A. (2024). Enhancing functional properties of rice starches through hydroxypropylation for development of reduced-fat white sauces. Food Chemistry, 446, 138860. https://doi.org/10.1016/J.FOODCHEM.2024.138860
Moin, A., Hosseini, E., Smaoui, S., & Varzakas, T. (2025). Research progress on lentil-based composite flours: physicochemical, techno-functional properties, and high-performance food applications. Quality Assurance and Safety of Crops & Foods, 17(2), 232–250.
Moin, A., Zaid, M., Moin, M., & Giuffrè, A. M. (2024). Consumer Acceptance and Sensory Properties of Wheat-Millet Composite Biscuits Fortified with Moringa oleifera and Camellia sinensis Leaves Powder. Current Research in Nutrition and Food Science Journal, 12(2).
Naz, A., Razzaq, K., Raza, N., Hussain, M., Mujtaba, A., Afzal, M. I., Umer, M., Alsuhaibani, A. M., Al-Shawi, A. H., Umar, M., Mushtaq, Z., Imran, M., & AL JBawi, E. (2023). Evaluation of enzymatic and non-enzymatic antioxidant potential of sprouted indigenous legumes from Pakistan. International Journal of Food Properties, 26(1), 1230–1243. https://doi.org/10.1080/10942912.2023.2207778
Onwuka, Q. I., Chinma, C. E., Ezeocha, V. C., Otegbayo, B., Oyeyinka, S. A., Adebo, J. A., Wilkin, J., Bamidele, O. P., & Adebo, O. A. (2024). Short-term germinated legume flours as functional ingredients in food products. Journal of Food Science, 89(10), 6070–6085. https://doi.org/10.1111/1750-3841.17334
Sattar, D. e. shahwar, Ali, T. M., & Hasnain, A. (2017). Effect of germination on enzymatic, functional and bioactive attributes of different Pakistani legume cultivars. Journal of Food Measurement and Characterization, 11(4), 2076–2086. https://doi.org/10.1007/S11694-017-9591-5/TABLES/6
Sattar, D. e. shahwar, Fauqiha, A. tul, Mohamed, M., Ali, T. M., & Hasnain, A. (2021). Comparative study on effects of adding germinated and non-germinated legumes on bioactive components, antioxidant, textural and sensory characteristics of cereal flakes. Legume Science, 3(1), e68. https://doi.org/10.1002/LEG3.68
Sattar, D.-S., Ali, T. M., & Hasnain, A. (2015). Effect of Different Techniques on Germination Efficacy and Antioxidant Capacity of Indigenous Legumes of Pakistan. Journal of Basic & Applied Sciences, 11, 348–353. https://doi.org/10.6000/1927-5129.2015.11.50
Sharanagat, V. S., Singh, L., & Nema, P. K. (2022). Approaches for development of functional and low gluten bread from sorghum: A review. Journal of Food Processing and Preservation, 46(11), e17089. https://doi.org/10.1111/JFPP.17089
Sokrab, A. M., Mohamed Ahmed, I. A., & Babiker, E. E. (2012). Effect of germination on antinutritional factors, total, and extractable minerals of high and low phytate corn (Zea mays L.) genotypes. Journal of the Saudi Society of Agricultural Sciences, 11(2), 123–128. https://doi.org/10.1016/J.JSSAS.2012.02.002
Spence, C., Wan, X., Woods, A., Velasco, C., Deng, J., Youssef, J., & Deroy, O. (2015). On tasty colours and colourful tastes? Assessing, explaining, and utilizing crossmodal correspondences between colours and basic tastes. Flavour 2015 4:1, 4(1), 1–17. https://doi.org/10.1186/S13411-015-0033-1
Wang, S., Xu, X., Wang, S., Wang, J., & Peng, W. (2022). Effects of Microwave Treatment on Structure, Functional Properties and Antioxidant Activities of Germinated Tartary Buckwheat Protein. Foods, 11(10), 1373. https://doi.org/10.3390/FOODS11101373/S1
Wunthunyarat, W., Seo, H. S., & Wang, Y. J. (2020). Effects of germination conditions on enzyme activities and starch hydrolysis of long-grain brown rice in relation to flour properties and bread qualities. Journal of Food Science, 85(2), 349–357. https://doi.org/10.1111/1750-3841.15008
Zhao, H., Zhang, B., Xiang, K., Li, D., Liu, C., Niu, L., Ma, Y., Xiao, Y., & Wang, H. (2026). Multiscale pore drives mechanical properties and storage stability in peach crisps. Journal of Food Engineering, 404, 112759. https://doi.org/10.1016/J.JFOODENG.2025.112759
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